[en] Ionizing radiation induced DNA damage may lead to mutation, cancer, and cellular death. Present study was carried out to elucidate the DNA protective ability of a formulation (G-003 M), a combination of Podophyllotoxin and Rutin hydrate, in murine blood and bone marrow cells under in vivo condition. The formulation was administered intramuscularly to mice 1 h prior to "6"0Co gamma irradiation exposure. Mice were assigned to groups that received one of the following treatments: Un-treated, G-003 M alone, 9 Gy irradiated and G-003M pre-administration followed by 9 Gy radiation exposures. The levels of DNA damage response proteins; gamma-H2AX, p53BP1, phosphorATMser-1981, DNA-PK, Ku-80, Ligase-IV, MRE-11, Rad-50 and NBS-1 were studied by immunocytochemistry method at various time points