[en] In the first part of the work, geometrically and radiochemically characterized standards (phantoms) imitating the plant tissues and allowing the exact quantification of visualised radioindicator in plant tissues were designed and prepared within the study of visual and analytical characteristics of used positron emission tomograph (microPET system) commercially developed for animal objects at visualization of thin objects. Individual experiments carried out by exposure of excised leaves of tobacco (Nicotiana tabacum L.) or radish (Raphanus sativus L.) in solutions of 2-deoxy-2-fluoro-D-glucose labelled with positron emitter "1"8F (2-["1"8F]FDG) containing 10-, 100-, or 1000-times higher concentrations of D-glucose (c_g_l_u) in comparison with the original 2-["1"8F]FDG solution showed that the significant changes in visualisation of 2-["1"8F]FDG distribution as well as in chemical portion of 2-["1"8F]FDG within the leaf blade were observed as result of increased c_g_l_u. In the experiments with the whole plants of tobacco or radish exposed in 2-["1"8F]FDG solution through the root system, only minimal translocation of "1"8F radioactivity into the above-ground parts of plants, also in the case of increased c_g_l_u, was observed, which suggest the role of root system as a selective barrier of 2-["1"8F]FDG transport from roots to the above-ground parts. On the basis of mentioned knowledge and analytical approaches (application of prepared phantoms), the dynamic study of 2-["1"8F]FDG uptake and transport within the excised leaf of tobacco or whole radish plant was carried out. The description of these processes was realized through the 3D PET images and through the quantification of 2-["1"8F]FDG distribution within the chosen regions of interest from the point of view of accumulated "1"8F radioactivity (in Bq) or amount of D-glucose (in μg) as well. Application of methods of multivariate analysis allows to found the similarities between studied objects mainly from the point of view of processes characterising the 2-["1"8F]FDG uptake and transport in plant tissues, as well as the relationships between the parameters defining the design or initial conditions of experiments and variables obtained within the microPET analysis or direct measurement of radioactivity. Obtained results of mentioned experiments and analyses suggest on the possibility of application of used microPET system in non-invasive in vivo imaging and in quantification of uptake and transport of 2-["1"8F]FDG in plant tissues under real-time conditions. (author)