[en] The spread of anthropogenic radioisotopes in the environment comes from nuclear tests and accidents that occurred in the past, which justifies constant monitoring, to guarantee the safety and control of the activities that involve these applications. Brazil, when exporting some food products, depending on the country of origin, a radiometric report is required, especially for sugar that has high world consumption and the possibility of contamination of the population, when radioisotope is present. Therefore, the investigation of the levels of Cs-134 and Cs-137 in sugar matrices is necessary to predict radioecological emergency situations. The National Nuclear Energy Commission, the agency that oversees nuclear applications in Brazil, With Resolution No. 102 of December 22, 2010 approved regulatory positions, including levels of action for food control, recommended to restrict the marketing of food products in Brazil. General, whose specific activity is higher than the limit of 1.0 kBq / kg for the said isotopes of cesium independently. Aiming for improvements in the analyzes that are already performed in the Laboratory of Radioecology and Environmental Control of the Department of Nuclear Energy of the Federal University of Pernambuco to assurance the quality of sugar marketed in Brazil, a standard procedure for the analysis of these radionuclides in this matrix was determined. High resolution gamma spectrometry with non-destructive analysis was used to perform the tests. Priority was given to parameters that directly influence the qualitative and quantitative analysis of these radioactive elements, such as calibration in energy and efficiency, resolution, influence of self-absorption, counting statistics directly associated with the time of analysis, influence of background radiation and geometry analysis. (author)