[en] Phanerochaete chrysosporium is the most potent fungus that plays a role in the delignification process because it produces enzyme lignin peroxidase (LiP) and manganese peroxidase (MnP). To enhance the ability of lignolytic activity carried out genetic improvement with gamma ray irradiation. Information on the genetic alteration due to irradiation of the Phanerochaete chrysosporium fungus is indispensable in order to improve the ability of the isolates for the bioremediation process. This study aimed to detect the mutant of P. chrysosporium fungus from gamma ray irradiation at doses of 250-2000 Gy using Random Amplified Polymorphic DNA (RAPD) marker. The seven primers of RAPD are used to amplify the DNA genome. The results showed that irradiation dose influenced on P. chrysosporium viability. D₅₀ value at 1000 Gy dose. Among the 7 primary, only 3 primers, namely OPA-1, OPA-4, and OPD-6 which yields polymorphic bands to analyze the mutation results at a dose of 500; 750; 1000; and 2000 Gy. The DNA-RAPD profile showed a high genetic variation between the irradiated isolate and the control isolate (0 Gy) with the 3-5 cluster formation. Dendrogram analysis showed the coefficient of similarity between 0.60-0.73. These results revealed that RAPD techniques can be easily used to detection on DNA mutation by irradiation. (author)