[en] ⁹³Mo is an important long-lived radionuclide in nuclear waste, and is required to be measured during the characterization of decommissioning waste. However, no commercial ⁹³Mo solution is available to be calibrated and used as standard in the analysis of nuclear waste. This work presents a method for separation of ⁹³Mo from Nb metal used in cyclotron as a target holder and irradiated with protons for long time. The separation of ⁹³Mo from Nb matrix was implemented by combination of precipitation and chromatographic separation. The Nb matrix was first removed by precipitating oxides-hydroxides of Nb (e.g. Nb₂O₅) and then by Fe(OH)₃ co-precipitation; Mo in the solution was purified using an alumina (Al₂O₃) column. A decontamination factor of ca. 10⁵ was achieved for Nb. A pure carrier-free ⁹³Mo solution was successfully prepared, and the ⁹³Mo purity was verified by liquid scintillation spectrometry. (author)