[en] Highlights: • Radionuclides-resistant A. sydowii and T. harzianum are isolated from radionuclides' contaminated soils. • The maximum tolerance to Eu(III) concentration of A. sydowii and T. harzianum reach 3000 mg/L and 3500 mg/L, respectively. • A. sydowii and T. harzianum show efficient accumulation of Eu(III) from wastewater. • The intercellular and extracellular morphologies of A. sydowii and T. harzianum changes obviously under Eu(III) stress. - Abstract: Radionuclides-resistant filamentous fungi were isolated from radionuclides' contaminated soils. Effects of contact time, mycelia dosage, pH, ionic strength and thiol compounds on ^152+154Eu(III) accumulation on two kinds of filamentous fungi (Aspergillus sydowii and Trichoderma harzianum, denoted as A. sydowii and T. harzianum, respectively) were investigated by batch techniques. The maximum tolerance to Eu(III) concentration of A. sydowii and T. harzianum reached 3000 mg/L and 3500 mg/L, and the Eu(III) accumulation on A. sydowii and T. harzianum can be fitted better with the pseudo-second-order kinetic model, respectively. Filamentous fungi were characterized by FT-IR and acid base titrations, and morphological structures of mycelia changed obviously under Eu(III) stress by SEM and TEM analysis. The results suggested that filamentous fungi could play an important role in the migration and transformation of radionuclides in the environment.