[en] Nanowires of polytetrakis(o-aminophenyl)porphyrin (PTAPPNW) were fabricated by electrochemical polymerization with the cyclic voltammetric method in anodic aluminum oxide (AAO) membranes. The glassy carbon electrode (GCE) modified by PTAPPNW, single-walled carbon nanotubes (SWNT) and Nafion as a binder was investigated with voltammetric methods in a phosphate buffer saline (PBS) solution at pH 7.4. The PTAPPNW + SWNT + Nafion/GCE exhibited strongly enhanced voltammetric and amperometric sensitivity towards hydrogen peroxide (H₂O₂), which shortened the response time and enhanced the sensitivity for H₂O₂ determination at an applied potential of 0.0 V by amperometric method. The PTAPPNW + SWNT + Nafion/GCE can be used to monitor H₂O₂ at very low concentrations in biological pH as an efficient electrochemical H₂O₂ sensor