[en] The aim of the present study was to isolate the active compound against Candida albicans from the methanolic extract of aerial part of Mollugo pentaphylla Linn. and to peruse in vivo animal model of oral candidiasis. The study was designed to quantify the oral candida infection with different statistical approach and histopathological observation of infected tongue on treatment with 100 µg/mL of the isolated compound. The significance of age, body weight and tongue weight was also correlated with disease treatment on either sex of animal. The isolated active compound was characterized as stigmasterol (EX-1) based on FTIR, DSC, ¹H NMR, ¹³C NMR and mass spectral analysis. An in silico docking approach predicted strong negative binding energy efficacy of stigmasterol with C. albicans PKh Kinase protein unveiled an in-visual perception for anticandidal efficacy.