[en] In this work, regenerated silk fibroin (SF)/poly-L-lactic acid (PLLA) composite electrospun fibers were successfully prepared by electrospinning method with single and double syringe systems. Furtherly, we applied simultaneous and alternative spinning when using double syringe systems. The morphologies of the obtained silk/PLLA hybrid nanofibers were characterized by scanning electron microscope (SEM). Fourier transform infrared spectroscopy (FTIR) showed that PLLA has been well incorporated into the SF electrospun fibers using double syringes. Furthermore, the tensile test results indicate the tensile strength and Young's modulus of the silk/PLLA hybrid nanofibers webs were improved by both single and double syringe system. Moreover, the cell adhesion ratios of simultaneous silk/PLLA nanofibers were much higher than that of alternative silk/PLLA nanofibers, and even higher than pure silk nanofibers. Therefore, simultaneous spinning using double syringe system is a potential method for fabrication of nanofiber scaffold.