[en] Peripheral blood that had been exposed to different doses of carbon ion beams and X-rays in female patients with breast cancer and cervical cancer was analyzed to observe the changes in lymphocyte subsets. The peripheral venous blood from women with cervical cancer (5 patients) and breast cancer (5 patients) was irradiated with carbon ion beams at 1, 2 GyE, X-rays at 1, 2 Gy, and 0 Gy (control), respectively, to test the lymphocyte subgroups. Using independent sample t-test to analyze the changes of CD3⁺, CD8⁺, CD4⁺, NK cells, B cells, and CD4⁺/CD8⁺ in the two in-vitro blood samples, respectively. The results showed that there were no significant differences (p > 0.05) in the values in in-vitro blood of lymphocyte subgroup after exposure to carbon ion beams and X-rays at different doses. The CD4⁺ value increased with the dose in the breast cancer group and decreased in the cervical cancer group when irradiated with high linear-energy-transfer (LET) carbon ion beams. The NK cell value increased with the dose in the breast cancer group and decreased in the cervical cancer group when irradiated with 6 MV X-rays. The NK cell value was higher in 1 GyE dose group than that in the control group, while lower in 2 GyE dose group when irradiated with carbon ion beams. These results indicate that the in-vitro peripheral blood of the most common two malignant tumors in female reproductive system irradiated with high LET carbon ions and low LET 6 MV X-rays shows different immune response. (authors)