[en] Inadequate tissue oxygenation affects cancer treatment and results in radio and chemo-therapy resistance. Evaluation of Oxygen deficiency is necessary to assure successful treatment. It was reported that positron emission tomography (PET) imaging with radioisotope could be used as molecular markers to detect tumor hypoxia. This method is based on the physics of 2γ annihilation. However, the 3γ annihilation yield is governed by the positronium formation as well as in quenching rates could be used as a new PET molecular imaging modality. This work describes the preparations and techniques that used the measurements of oxygen concentration in the biological samples. This work's influence is that it helps to identify the best methods for the preparation of biological samples with different oxygen concentrations. Besides, to develop a configuration system for the determination of the 3γ yield using high-purity germanium (HPGe) detectors to investigate the measurement of 3γ imaging. The results showed that 3γ annihilation with different oxygen concentrations could illustrate the oxygenation in blood or other biological models. Even though many diverse disciplines are involved in this work, each possible technique's advantages and disadvantages have yet to be concisely summarized. This work provides sufficient background to examine the clinical applications of the 3γ PET technique and compare this non-invasive method with existing oxygen measurement devices. (Author)