[en] Toll-like receptors (TLRs) are a family of innate immune receptors that recognize molecular patterns associated with microbial pathogens (PAMP) and induce antimicrobial immune responses. Here we report the expression of TLR2, Myeloid differentiation primary response gene 88 (Myd88) and Interleukin 1beta (IL1β) from the Gouramy an economically freshwater fish in Indonesia. Upon stimulation with Aeromonas hydrophila, the TLR2 expression was obviously up-regulated in spleen and liver, Myd88 expression up-regulated in spleen and kidney, and IL1β expression was up-regulated in a spleen, kidney, and liver. The highest expression of TLR2 was in liver or hepatopancreas, MyD88 in kidney and IL1β was in the spleen. These findings together highlighted the contribution of TLR2 in augmenting innate immunity in fish and indicated its important role in immune surveillance of various organs during the pathogenic invasion. This study will enrich the information in understanding the innate immune mechanism in fish and will be helpful in developing preventive measures against infectious diseases in fish. (paper)