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AbstractAbstract
[en] A hypothesis is proposed according to which E. coli possesses an inducible DNA repair system. This hypothetical repair, which we call SOS repair, is manifested only following damage to DNA, and requires de novo protein synthesis. SOS repair in E. coli requires some known genetic elements: recA+, lex+ and probably zab+. Mutagenesis by ultraviolet light is observed only under conditions of functional SOS repair: we therefore suspect that this is a mutation-prone repair. A number of phenomena and experiments is reviewed which at this point can best be interpreted in terms of an inducible mutagenic DNA repair system. Two recently discovered phenomena support the proposed hypothesis: existence of a mutant (tif) which, after a shift to elevated temperature, mimicks the effect of uv irradiation in regard to repair of phage lambda and uv mutagenesis, apparent activation of SOS repair by introduction into the recipient cell of damaged plasmid or Hfr DNA. Several specific predictions based on SOS repair hypothesis are presented in order to stimulate further experimental tests. (U.S.)
Original Title
UV radiation
Primary Subject
Source
Prakash, L. (ed.); p. 128-142; 1974; Charles C Thomas Publisher; Springfield, IL; 6. international conference on environmental toxicity: molecular and environmental aspects of mutagenesis; Rochester, New York, USA; 4 Jun 1973
Record Type
Book
Literature Type
Conference
Country of publication
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