[en] ⁶⁷Ga accumulates in various malignant tumors and parenchymatous tissues. It was found to be associated with the soluble fraction of lysosomes. The present work investigates the mechanism of ⁶⁷Ga accumulation in normal liver cells. Lysosomes were isolated from rat liver after intravenous injection of carrier free ⁶⁷Ga. The soluble lysosomal was obtained by sonication followed by centrifugation at 105,000 xg for 2 hrs. After staining with Coomassie blue 18 separated protein bands were apparent. ⁶⁷Ga distribution within the gels was assessed by direct counting of radioactivity in gel slices. A considerable amount of the intralysosomal protein bound radioactivity migrated with a relative mobility of 0.36 corresponding to a protein band of molecular weight 85,000 - 90,000. This peak corresponded to the peak of ⁶⁷Ga labelled purified transferrin in control gels. These data were confirmed by immunoelectrophoresis combined with autoradiography: within the soluble lysosomal fraction a slight transferrrin line could be identified. We conclude that ⁶⁷Ga which is transported in the blood by transferrin and taken up by the hepatic cell through endocytosis is accumulated in the lysosomes associated with transferrin and its degraded fragments. (orig./GSE)