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AbstractAbstract
[en] A routine and automatized methodology for thyroxine (T4) and thyrotropin (TSH) radioimmunoassay (RIA) using dried blood samples on filter paper is described. Five mm diameter dots were prepared. One eluted dot, corresponding to 4 μl of plasma, was used for T4-RIA while two were necessary for TSH-RIA. Reference filter papers were introduced in each assay for quality control. In a preliminary study on 1903 newborns, samples were obtained, generally between the 5th-7th day. Mean dot T4 was 7.38 +- 2.5 μg/dl. Mean dot TSH was 11.83 +- 9.1 μU/ml, the equation of the regression line between dot TSH (y) and serum TSH (x) being Y = 10.29 + 0.623x. (orig.)
[de]
Es wird eine automatisierte Routinemethode fuer den Radioimmunoassay (RIA) mit Thyroxin (Th) und Thyrotropin (TSH) unter Verwendung von Trockenblutproben auf Filterpapier beschrieben. Die Proben wurden als Kreise mit 5 mm Durchmesser vorbereitet. Ein eluierter Kreis, entsprechend 4 μl Plasma, wurde fuer T4-RIA benutzt, waehrend fuer TSH-RIA zwei notwendig waren. Zur Qualitaetskontrolle wurden in jedem Test Filterpapiere benutzt. In einer Vorstudie bei Personen von Jahrgang 1903 wurden Proben entnommen, i.a. zwischen dem 5. und 7. Tag. Hauptkreis T4 war 7,38+-2,5 μg/dl. Hauptkreis TSH war 11,83+-9,1 μU/ml, die Gleichung der Regressionslinie zwischen Kreis TSH (y) und Serum TSH (x) lautet y = 10,29 + 0,623x. (orig.)Primary Subject
Source
1977; 11 p; International symposium on radioimmunoassay and related procedures in medicine; Berlin, Germany, F.R; 31 Oct - 4 Nov 1977; IAEA-SM--220/55; 3 figs.; 10 refs.
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Miscellaneous
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Conference
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AMINO ACIDS, ANIMALS, BIOLOGICAL MATERIALS, BODY FLUIDS, CARBOXYLIC ACIDS, CONTROL, DISEASES, ENDOCRINE DISEASES, HORMONES, ISOTOPE APPLICATIONS, MEDICINE, ORGANIC ACIDS, ORGANIC COMPOUNDS, ORGANIC HALOGEN COMPOUNDS, ORGANIC IODINE COMPOUNDS, PEPTIDE HORMONES, PITUITARY HORMONES, THYROID HORMONES, TRACER TECHNIQUES
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