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Miller, J.E.; Muller, R.N.; Seegers, P.
Radiological and Environmental Research Division annual report: Ecology, January--December 19761976
Radiological and Environmental Research Division annual report: Ecology, January--December 19761976
AbstractAbstract
[en] The process of photosynthesis in green plants is the major determinant of crop yield. Although the effects of air pollutants, such as sulfur dioxide, on photosynthesis has been studied, many unsolved questions remain. This is especially true with regard to reduction of photosynthetic rate under conditions of chronic exposure causing little or no visible injury. It was the purpose of these studies to develop techniques suitable for measuring photosynthetic rates of field-grown plants without dramatically altering the microenvironment of the plants. Gross photosynthetic rates of soybeans (Glycine max. cv. Wayne) in the field were measured by exposing a small section of representative leaves for 30 seconds to 14CO2 in a normal atmospheric mixture by a technique similar to that of Incoll and Wright. A 1-cm2 section of the area exposed to 14CO2 is punched from the leaf and processed for liquid scintillation counting. Since the treatment period is of such short duration, there is little photorespiratory loss of 14CO2, and thus, the amount of 14C fixed in the leaf can be related to the gross photosynthetic rate. Other parameters measured during the course of these experiments were stomatal resistance, light intensity, leaf water potential, and air temperature
Original Title
14CO2 tracer study
Primary Subject
Source
Argonne National Lab., Ill. (USA); p. 3-5; 1976; p. 3-5
Record Type
Report
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