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[en] The work describes nonstandard application of filters from ASS-500 station for the determination of the element content in the samples collected by PIXE method. Determination of gamma radioactive isotopes and alpha radioactive plutonium is also reviewed. Authors conclude that ASS-500 workstation allows collection of representative samples from the ground level air. These samples are suitable for the complex analysis of industrial pollution
[en] The mechanical unfolding of two proteins belonging to the immunoglobulin superfamily, fibronectin (an extracellular matrix protein) and contactin (a neuronal adhesion protein), was studied by means of atomic force microscopy (AFM). The mean unfolding forces and characteristic lengths describing unfolding events of two types of the immunoglobulin module observed for contactin were compared with results obtained for fibronectin. The results showed that the FnIII-type domain present in both proteins, i.e. in contactin and fibronectin, requires a similar force of about 100 pN to be unfolded. However, the IgC2-type domains of contactin, normally remaining intact in view of the intra-domain disulfide bonds, reveal rather lower stability in the presence of the reducing agent. The force needed to unfold a single IgC2 domain was calculated and established to be about 70 pN. Initially, natural human fibronectin was chosen only as a reference protein for studies of contactin unfolding force values. However, interesting results were obtained and used as a reference in further analysis of the contactin unfolding pathway. Two characteristic length values were obtained for the FnIII domain type of both studied proteins; thus for both domains the ability to unravel in two different pathways was concluded
[en] This report describes the Henryk Niewodniczanski Institute of Nuclear Physics in Cracow activities in years 2003 and 2004. The Report consist of two main parts: first describes the scientific program activity and its progress while the second one the financial details of their realization. The list of published papers and other information about staff, conferences and educational activities are also given
[en] Sulphur plays a vital role in every human organism. It is known, that sulphur-bearing compounds, such as for example cysteine and glutathione, play critical roles in development and progression of many diseases. Any alteration in sulphur's biochemistry could become a precursor of serious pathological conditions. One of such condition is prostate cancer, the most frequently diagnosed malignancy in the western world and the second leading cause of cancer related death in men. The purpose of presented studies was to examine what changes occur in the nearest chemical environment of sulphur in prostate cancer cell lines in comparison to healthy cells. The Extended X-ray Absorption Fine Structure (EXAFS) spectroscopy was used, followed by theoretical calculations. The results of preliminary analysis is presented.