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Journal Article
Journal
Nuclear Technology; v. 20(2); p. 79-85
Country of publication
ALGORITHMS, CONTROL SYSTEMS, CORRELATION FUNCTIONS, DATA PROCESSING, DIGITAL SYSTEMS, DISTURBANCES, ELECTRONIC EQUIPMENT, HBWR REACTOR, IN CORE INSTRUMENTS, ON-LINE CONTROL SYSTEMS, POWER DENSITY, REACTOR CONTROL SYSTEMS, REACTOR INSTRUMENTATION, REACTOR NOISE, SPECTRA, STOCHASTIC PROCESSES, TRANSFER FUNCTIONS
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AbstractAbstract
[en] Cell cycle kinetics and radiation response under hypoxic conditions were analyzed with human cells of the line NHIK 3025. The cells were either kept on continuous exponential growth by frequent reculturing, of went through log and plateau phase for each passage (recultured weekly). The cell cycle time for weekly recultured populations in early log phase was shorter than for cells in continuous exponential growth. Cells in continuous exponential growth were more sensitive to radiation than cells in log phase. The difference in sensitivity was not due to partial synchrony of weekly recultured populations. (Auth.)
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Journal Article
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Acta Radiol., Oncol., Radiat. Phys., Biol; v. 17(4); p. 319-330
Country of publication
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AbstractAbstract
[en] The effect of X-irradiation on the cell cycle progression of synchronized populations of the human cell line NHIK 3035 has been studied in terms of the radiation-induced delay of DNA replication and cell division. Results were obtained by flow cytometric measurements of histograms of cellular DNA content and parallel use of conventional methods for cell cycle analysis, such as pulse labelling with (3H) thymidine and counting of cell numbers. The two sets of methods were generally in good agreement, but the advantages of employing two independent techniques are pointed out. Irradiation was found to have a minor influence on DNA replication. As compared with unirradiated populations, half-completed DNA replication was 20-30 min delayed in populations given 580 rad in mid-G1 or 290 rad in early S. Cell cycle progression was markedly delayed in G2. The sensitivity induction of this delay was 0.6 min/rad for populations irradiated in mid-G1, and 1.4 min/rad for populations irradiated in early S. (author)
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Journal Article
Literature Type
Numerical Data
Journal
Cell and Tissue Kinetics; ISSN 0008-8730;
; v. 12(1); p. 43-57

Country of publication
AZINES, BIOLOGICAL EFFECTS, DATA, DATA FORMS, ELECTROMAGNETIC RADIATION, HETEROCYCLIC COMPOUNDS, HYDROGEN COMPOUNDS, INFORMATION, IONIZING RADIATIONS, ISOTOPES, NUCLEIC ACID REPLICATION, NUCLEOSIDES, NUCLEOTIDES, NUMERICAL DATA, ORGANIC COMPOUNDS, ORGANIC NITROGEN COMPOUNDS, PYRIMIDINES, RADIATION EFFECTS, RADIATIONS, RIBOSIDES
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AbstractAbstract
No abstract available
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29 refs.
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Journal Article
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Bibliography
Journal
Energ. Nucl. (Milan); v. 20(7); p. 385-399
Country of publication
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AbstractAbstract
[en] The effect of single dose irradiation on the proliferation kinetics in a human malignant melanoma grown in the athymic mutant nude mouse was analysed. DNA-histograms were obtained with flow cytofluorometry. Percentage labelled mitoses curves were established by the use of conventional autoradiographic techniques. Changes in the fraction of clonogenic cells with time after irradiation were measured in vitro in soft agar. In non-irradiated tumours the fraction of cells in G1/G0, S and G2+M was 66, 21 and 13 per cent respectively. The median duration of G1, S, G2, M and Tsub(c) was 19.0 h, 13.3 h, 5.0 h, 1.0 h and 41.1 h, respectively. The growth fraction was calculated as 0.66 and the cell loss factor as 0.67. The growth fraction was increased after irradiation and the cell cycle time reduced, due to a shortening of G1. These effects were dose dependent and decreased with time after exposure, but were still present after the tumours had resumed a continuous volume growth. The rate of volume growth was slower for irradiated tumours than for non-irradiated tumours of the same size, due to a larger cell loss factor for the former. (Auth.)
Primary Subject
Record Type
Journal Article
Literature Type
Numerical Data
Journal
Acta Radiol., Oncol., Radiat. Ther., Phys., Biol; v. 19(4); p. 261-269
Country of publication
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INIS IssueINIS Issue
AbstractAbstract
[en] The anti-melanoma antibody 9.2.27 localizes to melanoma cells when administered i.v. to melanoma patients, but high doses of this antibody alone have no specific cytotoxic effect in vivo. To determine whether radiolabeled antibodies would exhibit specific antimelanoma cytotoxicity in vitro, cell survival curves were established for NCl-N892 human melanoma cells treated with 125I-labeled 9.2.27 monoclonal antibody. The binding capacity per cell was 5 X 10(5) molecules of 9.2.27 immunoglobulin G, and the association constant of binding was 10(10) M-1. Antibody preparations with specific radioactivities of 9-80 microCi/micrograms were used. Colony-forming ability after in vitro exposure to 125I-9.2.27 was determined by a 1-h antibody incubation at saturating concentrations, washing, and cell freezing for various exposure durations. Colony survival was dose dependent, varying with the radioactivity per cell and the exposure time. The survival curves demonstrated no shoulder effect and had a 37% incremental survival dose of 0.5-0.9 X 10(5) decays/cell. Selective killing of melanoma cells was demonstrated in experiments where NCl-N417 lung cancer cells were mixed with the melanoma cells prior to antibody treatment. The NCl-N417 cells did not express the melanoma-associated antigen, were more sensitive to conventional external irradiation than were the melanoma cells, and could easily be distinguished from them by different growth morphology. In spite of a growth advantage for the melanoma cells in the clonogenic assay, the antigen-negative lung cancer cells selectively survived the treatment and were the only surviving cells after 15 days of exposure
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Journal Article
Journal
Country of publication
ANIMAL CELLS, ANTIBODIES, BETA DECAY RADIOISOTOPES, BODY, DAYS LIVING RADIOISOTOPES, DISEASES, ELECTRON CAPTURE RADIOISOTOPES, GLOBULINS, INTERMEDIATE MASS NUCLEI, IODINE ISOTOPES, ISOTOPE APPLICATIONS, ISOTOPES, NEOPLASMS, NUCLEI, ODD-EVEN NUCLEI, ORGANIC COMPOUNDS, ORGANS, PROTEINS, RADIOISOTOPES, RESPIRATORY SYSTEM
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
AbstractAbstract
[en] The radiolabeled anti-T cell antibody T101 can be used for specific tumor localization, but unlabeled T101 produces limited cytotoxicity in patients. We studied the in vitro cytotoxic effects of T101 labeled with 125I, a radionuclide known for its short-range, high-linear-energy electrons. We showed that 125I-T101 could be readily prepared at high specific activity with high immunoreactivity. Human malignant T cell lines HUT 102, MOLT-4, and HUT 78 were found to differ in the number of T65 determinants (the antigen recognized by T101) and the sensitivity to external x-ray radiation, which were of significance for the cytotoxicity of 125I-T101 in vitro. The cytotoxic effects of 125I-T101 were also found to be dose dependent and increased with exposure time under frozen conditions. As controls, unlabeled T101 had no cytotoxic effect, while free Na 125I or the 125I-labeled irrelevant antibody 9.2.27 exerted minor cytotoxicity. In HUT 102 and MOLT-4, more than 3 logs cell killing was achieved within four weeks. Because considerable cytotoxicity was demonstrated in vitro by 125I-T101 on T65-positive malignant cells, and because low-dose 111In-T101 can be used successfully for tumor localization, future trials using 125I-T101 at high specific radioactivity may improve therapeutic results in patients with T65-positive malignancies
Primary Subject
Record Type
Journal Article
Journal
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ANIMAL CELLS, ANTIBODIES, BETA DECAY RADIOISOTOPES, BIOLOGICAL MATERIALS, BLOOD, BLOOD CELLS, BODY FLUIDS, CONNECTIVE TISSUE CELLS, DAYS LIVING RADIOISOTOPES, DISEASES, ELECTRON CAPTURE RADIOISOTOPES, HEMIC DISEASES, INTERMEDIATE MASS NUCLEI, IODINE ISOTOPES, ISOTOPES, LEUKOCYTES, MATERIALS, NEOPLASMS, NUCLEI, ODD-EVEN NUCLEI, RADIOISOTOPES, SOMATIC CELLS
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
Lindmo, T.; Boven, E.; Cuttitta, F.; Fedorko, J.; Bunn, P.A. Jr.
Nuclear medicine in research and practice1984
Nuclear medicine in research and practice1984
AbstractAbstract
No abstract available
Source
Schmidt, H.A.E.; Vauramo, E. (eds.); Nuklearmedizin. Supplementum; no. 21; 950 p; ISBN 3-7945-1031-3;
; 1984; p. 807-810; Schattauer; Stuttgart (Germany, F.R.); European nuclear medicine congress; Helsinki (Finland); 13-17 Aug 1984; Published in summary form only.

Record Type
Book
Literature Type
Conference
Country of publication
BETA DECAY RADIOISOTOPES, BETA-MINUS DECAY RADIOISOTOPES, CONTROL, DAYS LIVING RADIOISOTOPES, ELECTRON CAPTURE RADIOISOTOPES, IMMUNOLOGY, INDIUM ISOTOPES, INTERMEDIATE MASS NUCLEI, IODINE ISOTOPES, ISOMERIC TRANSITION ISOTOPES, ISOTOPES, MINUTES LIVING RADIOISOTOPES, NUCLEI, ODD-EVEN NUCLEI, RADIOISOTOPES
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AbstractAbstract
[en] Conjugates of monoclonal antibodies with radioactive isotopes, drugs or toxins have great potential for specific radiolocalization and inactivation of tumor cells. Because the conjugation procedure may adversely alter the antibody, quality control procedures must be applied to determine important characteristics of the conjugated antibody. One such property is how much of the conjugated antibody is able to bind to the relevant antigen. Based on theoretical considerations, we have developed a binding assay for radiolabeled monoclonal antibodies in which the fraction of immunoreactive antibody is determined by linear extrapolation to conditions representing infinite antigen excess. This ensures that the true value of the immunoreactive fraction is obtained, as opposed to the apparent immunoreactive fraction determined under conditions of limited antigen excess. The described assay is based on a double-inverse plot of the binding data which may be considered a modification of the Lineweaver-Burk plot. We established the method using 125I- and 111In-labeling of the 2 monoclonal antibodies T101 and 9.2.27 which currently are undergoing radioimaging trials at the National Cancer Institute. For properly performed conjugation procedures, immunoreactive fractions of about 0.9 were obtained, but a prolonged chloramine-T reaction for 125I-labeling resulted in an immunoreactive fraction of only 0.6. Due to its principle of determining binding at infinite antigen excess, the present method is quite insensitive to variation in the actual amounts of cells and antibody used, as well as the incubation time. (Auth.)
Primary Subject
Record Type
Journal Article
Journal
Journal of Immunological Methods; ISSN 0022-1759;
; v. 72(1); p. 77-89

Country of publication
AMINES, ANTIBODIES, BETA DECAY RADIOISOTOPES, CONTROL, DAYS LIVING RADIOISOTOPES, DIAGNOSTIC TECHNIQUES, DRUGS, ELECTRON CAPTURE RADIOISOTOPES, INDIUM ISOTOPES, INTERMEDIATE MASS NUCLEI, IODINE ISOTOPES, ISOMERIC TRANSITION ISOTOPES, ISOTOPES, LABELLED COMPOUNDS, MATERIALS, MEDICINE, MINUTES LIVING RADIOISOTOPES, NUCLEI, ODD-EVEN NUCLEI, ORGANIC CHLORINE COMPOUNDS, ORGANIC COMPOUNDS, ORGANIC HALOGEN COMPOUNDS, RADIOACTIVE MATERIALS, RADIOISOTOPES
Reference NumberReference Number
INIS VolumeINIS Volume
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Sairanen, R.; Persson, P.; Hedemann Jensen, P.; Lindmo, T.
Nordisk Kernesikkerhedsforskning, Roskilde (Denmark)2006
Nordisk Kernesikkerhedsforskning, Roskilde (Denmark)2006
AbstractAbstract
[en] NKS research work during the years 2002-2005 and its results have been evaluated against a set of criteria defined by the NKS Board. The evaluation encompassed the NKS-R (reactor safety) and NKS-B (emergency preparedness) programs and was conducted by two persons per program. The mode of work of the two evaluation teams was adapted to the special conditions of the program at hand, one being aimed more at the nuclear industry and the other at a more academic surrounding; in both cases, however, with great involvement of relevant national authorities. The findings of the evaluators are presented in this report. Financing and participating organizations, end users, deliverables, quality aspects, cost-benefit issues, time schedules, budgets and related issues are discussed. Finally, the sections on NKS-R and NKS-B, respectively, include conclusions and recommendations for future NKS work. (au)
Primary Subject
Source
Dec 2006; 116 p; ISBN 87-7893-208-4;
; Also available at http://www.risoe.dk/rispubl/NKS/nks-145.pdf

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