[en] An act published by the Government of Ontario by the Minister of Health to ensure public safety while subjected to the use of x-rays for the irradiation of human beings for therapeutic or diagnostic purposes

[en] By using poly(A)-selected RNA from Berne virus (BEV)-infected embryonic mule skin cells as a template, cDNA was prepared and cloned in plasmid pUC9. Recombinants covering a contiguous sequence of about 10 kilobases were identified. Northern (RNA) blot hybridizations with various restriction fragments from these clones showed that the five BEV mRNAs formed a 3'-coterminal nested set. Sequence analysis revealed the presence of four complete open reading frames of 4743, 699, 426, and 480 nucleotides, with initiation codons coinciding with the 5' ends of BEV RNAs 2 through 5, respectively. By using primer extension analysis and oligonucleotide hybridizations, RNA 5 was found to be contiguous on the consensus sequence. The transcription of BEV mRNAs was studied by means of UV mapping. BEV RNAs 1, 2, and 3 were shown to be transcribed independently, which is also likely--although not rigorously proven--for RNAs 4 and 5. Upstream of the AUG codon of each open reading frame a conserved sequence pattern was observed which is postulated to function as a core promoter sequence in subgenomic RNA transcription. In the area surrounding the core promoter region of the two most abundant subgenomic BEV RNAs, a number of homologous sequence motifs were identified

[en] The radiosensitivities and the kinetics for removal of radiation-induced DNA damage were compared for proliferative (P) and quiescent (Q) cells of the lines 66 and 67 derived from a mouse mammary adenocarcinoma. As determined from cell survival assays, the 66 and 67 Q cells were more radiosensitive than their 66 and 67 P counterparts. The rank order of their radiosensitivity was: 67 Q>66 Q≥67 P>66 P. Induction of radiation damage in the DNA of these cells, as measured by the alkaline elution technique, was identical for 66 and 67 P and Q cells. The repair of this DNA damage was biphasic for 66 and 67 P and Q cells. The half-times for the fast and slow repair phases in 66 Q cells were identical to those previously measured in 67 Q cells. The half-times of the fast and slow repair phases in 66 P cells were also identical to those previously measured in 67 P cells. However, the half-times for the fast and slow repair phases in 66 and 67 Q cells were longer than those measured in their 66 and 67 P counterparts. The 66 cell data are consistent with our previously published hypothesis that Q cells are more radiosensitive than their corresponding P cells because they repair their radiation-induced DNA damage slower. However, our results are not consistent with hypotheses that attempt to explain the radiosensitivity differences between lines 66 and 67 solely on the basis of measurable induction and repair of DNA damage. (orig.)

[en] Radiation damage from ²⁴¹Am to bone marrow cells was manifest in long-term bone marrow cultures (LTC) from offspring of mice radiocontaminated at 14th day of gestation (119, 479, 803, 1754 kBq ²⁴¹Am kg). Offspring were reared by their own contaminated mother for 3 weeks postnatal. LTC from these offspring were less able to support in vitro CFC proliferation than control LTC. This radiation damage persisted 71 weeks after radiocontamination in utero. Damage was observed at lower doses if ²⁴¹Am contamination occurred at foetal rather than adult ages. Radiation damage was observed only using LTC. After culturing LTC in 25% FCS and recharging the stromal adherent layer with bone marrow cell suspensions originating either from control offspring or from offspring contaminated with ²⁴¹Am in utero evidence was found that the proliferation capacity of haemopoietic cells was diminished. However, the nature of effects on the stromal elements is currently somewhat equivocal. Following in utero contamination stromal adherent cells appeared to support better production of in vitro CFC. (author)

[en] In the early 1980s, it was recognized by the FAO and the IAEA that a genetic sexing method for the Mediterranean fruit fly (medfly) would greatly improve the efficacy of the medfly sterile insect technique (SIT) and reduce its costs. These Proceedings summarize the research and development findings of the Agency's co-operators in the co-ordinated research programme to develop a genetic sexing method for the medfly. Great progress has been made in many aspects of medfly genetics. including the development of a number of genetic sexing strains. Contents: Genetics, Cytogenetics and Population Genetics. Genetic Sexing of Ceratitis Capitata by Morphological, Biochemical and other means. Recommendations. Refs, figs and tabs

[en] The electrophoretic variation of eight enzymes - β-hydroxy acid dehydrogenase (β-HAD); 6-phosphogluconate dehydrogenase (6-PGD); phosphoglucomutase (PGM); aldehyde oxidase (AO); malate dehydrogenase (MDH); alcohol dehydrogenase (ADH); α-glycerophosphate dehydrogenase (α-GPDH); and glutamate oxaloacetate transaminase (GOT) - was studied in Kenyan strains of the Mediterranean fruit fly (medfly), Ceratitis capitata (Wiedemann). The medflies were collected from ripe coffee berries in eight localities in the country. The berries were kept in paper cartons lined with sawdust in the insectary, and pupae were routinely recovered over a period of 14 days. Adults emerged in cages within the next 14 days, upon which they were used for gel electrophoresis. The Mdh locus was monomorphic, while only two alleles per locus were detected at the Ao, Adh, α-Gpdh and Got loci. Four different alleles were identified at the Pgm locus, six at the 6-Pgd locus, and seven at the β-Had locus. In addition, null β-Had and 6-Pgd heterozygotes were present in specimens from Kabete and Machakos. The mean heterozygosities for the two adequately sampled localities, Kabete and Machakos, were 0.143 and 0.100 respectively. The allele frequencies at the various loci were in agreement with Hardy-Weinberg expectations, except the β-Had locus in the Kabete population, where a significant deviation was observed. (author). 10 refs, 4 tabs

[en] Published in summary form only

[en] CT examinations of 11 consecutive patients with neurofibromatosis type 2 (NF-2) revealed non-tumoral intracranial calcified deposits in seven cases. Abnormal calcification of the choroid plexus was found in six cases. Calcification in the cerebellar hemispheres was observed in four cases. In two cases nodular calcifications on the surface of the cerebral hemispheres were detected. Our findings and the data in the literature show that non-tumoral calcifications of different locations can be regarded as part of the NF-2 syndrome. (orig.)

[en] The load and the stress of the hip joint are related to the density of bone, thickness of cartilage and pattern of the cancellous bone. The X-rays of the hip joint must provide all information for the calculation of the individual strain and stress. (orig.)

[en] Indium-111-labeled AUA1 tumor-associated monoclonal antibody raised against an antigen of colon adenocarcinoma was used to evaluate the effect of ionizing radiation on antibody uptake by the LoVo adenocarcinoma cell line grown as a xenograft in nude mice. Tumors were exposed to single doses of external X-irradiation of between 400 and 1600 cGy followed, 24 h later, by administration of specific or nonspecific antibody. Animals were sacrificed 3 days after antibody administration. At doses higher than 400 cGy, tumor uptake with both specific and nonspecific antibody was significantly increased. No difference in changes in tumor volume was observed between the groups receiving irradiation and the controls. Specific antibody uptake by tumors was always significantly higher than nonspecific having an approximate 4-fold binding advantage. Vascular permeability and the vascular volume of irradiated and control tumors was measured 24 and 72 h after irradiation, using iodine-125-labeled nonspecific antibody and labelling of the red blood cells in vivo with 99mTcO4. At doses higher than 400 cGy, vascular permeability in the tumor 24 h after irradiation was significantly increased (P less than 0.05), while the vascular volume decreased (P less than 0.001) compared to control values. However at 72 h after irradiation there was no difference between treated and control groups. The results obtained in this study suggest a potential value of external irradiation to increase monoclonal antibody uptake by tumors governed mainly by the increased vascular permeability of the tumor vasculature soon after the irradiation exposure