The effects of WAF and CEWAF on EROD activity in juvenile Atlantic cod
AbstractAbstract
[en] The affects of dispersed oil and hydrocarbons on Atlantic cod were examined in this study in which water accommodated fractions (WAF) of two types of oil were generated in a wave tank or in a baffled flask. Oil droplet sizes of the two systems were comparable. Chemically enhanced water accommodated fractions (CEWAF) of combinations of two oils and two dispersants were prepared in a similar manner. Juvenile Atlantic cod (Gadus morhua) were exposed to a broad range of concentrations of either WAF or CEWAF before being transferred to clean seawater. Periodic sampling of 5 fish confirmed the liver ethoxyresorufin-O-deethylas (EROD) activity. At the end of a 4 hour exposure, EROD activity was no different from the controls, but it was much higher after 20 hours at CEWAF concentrations above 2 percent v/v regardless of oil or dispersant type. Over the next 48 hours, EROD activity declined and did not return to control or pre-treatment levels even after 72 hours. EROD in livers from cod exposed to WAF was much higher after 24 hours at concentrations of 12 percent v/v or higher, reaching a maximum of 8 picomoles/mg protein/min. After 72 hours, EROD activity in WAF-exposed livers decreased to pre-treatment levels. The study showed that a brief 4 hour exposure to WAF and CEWAF can induce EROD activity in livers of juvenile cod and that the surge in enzyme activity can continue for 24-48 hours.
Primary Subject
Secondary Subject
Source
Fletcher, T. (Ontario Ministry of the Environment, Toronto, ON (Canada)); Holdway, D. (Univ. of Ontario Inst. of Technology, Oshawa, ON (Canada)) (comps.); Aquatic Toxicity Workshop, Toronto, ON (Canada). Funding organisation: Vale Inco Ltd., Sudbury, ON (Canada); Government of Ontario, Toronto, ON (Canada); Aquatox Testing and Consulting Inc., Guelph, ON (Canada); Teck, Toronto, ON (Canada); Stantec Consulting Ltd., Surrey, BC (Canada); Ryerson Polytechnic Univ., Toronto, ON (Canada); CropLife Canada, Toronto, ON (Canada); Environment Canada, Ottawa, ON (Canada); EcoMetrix Inc., Mississauga, ON (Canada); SGS Canada Inc., Mississauga, ON (Canada); ExxonMobil, St. John's, NL (Canada); Hatfield Consultants, North Vancouver, BC (Canada); Golder Associates Ltd., Toronto, ON (Canada); LUCAS, Thunderbay, ON (Canada); Pollutech Enviroquatics Ltd., Oakville, ON (Canada); Investigative Science Inc., Burlington, ON (Canada) (and others); 121 p; 2010; p. 33; 37. annual aquatic toxicity workshop: big cities, big challenges, great solutions: urbanization and environmental impacts; Toronto, ON (Canada); 3-6 Oct 2010; Available from the website of the Aquatic Toxicity Workshop at http://www.atw.ca/site_pages/2010/abstract_book_2010.pdf
Record Type
Miscellaneous
Literature Type
Conference
Country of publication
Descriptors (DEI)
Descriptors (DEC)
Reference NumberReference Number
Related RecordRelated Record
INIS VolumeINIS Volume
INIS IssueINIS Issue