Utilization of experimental mutagenesis to create high protein forms of field beans

To induce mutation variations F1, F2 and F3 seeds of the crossing Prelom and Tyrnovo 13 are utilized. Just before the sowing the seeds are treated independently or in combination with gamma rays (Cs 137) and ethylmethanesulfonate (EMS) solutions. It is established that the treatment of the seeds with mutagenes favours the variability in increasing of the protein content in the hybrid population. Highest number of transgressive forms characterized by both high protein content and good productivity is createdby the combined ttreatment of F2 seeds with gamma-rays and 0.15% EMS. The application of the hybridization and the experimental mutagenesis resulted in the creation of eleven lines characterized by protein content more than 28%. 8 refs., 3 tabs. (author)