Click chemistry mediated synthesis of prussian blue anchored carbon black for electrochemical detection of H₂, O₂

Hydrogen peroxide (H₂, O₂,) is one of the important reactive oxygen species, produced from oxygen metabolism in cells and is usually involved in cell growth and signal transduction. Level of H₂, O₂, is an important biological parameter in monitoring and maintaining the physiological balance of a living cell. Herein, prussian blue anchored carbon black (CB-PB) was synthesized via thiol-ene 'click chemistry' for the electrochemical detection of H₂, O₂, In CB-PB, PB rendered peroxidase-like activity while CB with large surface area and high conductivity efficiently enhanced the catalytic performance. Electrochemical studies, XRD, UV-visible and FTIR spectroscopy confirmed the anchoring of PB on the CB. The composite exhibited good catalytic performance toward H₂, O₂, detection with sensitivity of 620 μA/mM/cm²and detection limit of 0.61μM with a linear range of 0.01-10 mM (author)