Published 1980 | Version v1
Journal article Metadata-only

Effect of superoxide dismutase and chemical radioprotectors (cysteine and glutathione) on the radiation induced changes in the DNA molecule. 2

Creators

  • 1. Lodz Univ. (Poland)

Description

Results concerning the radioprotective action of superoxide dismutase (SOD) and of the chemical radioprotectors cysteine and glutathione on 60Co γ-irradiated DNA are presented. The highest reduction of the radiation yield for single breaks Gp1 was found in the cases of irradiation in the presence of chemical radioprotectors. By estimation of polynucleotide chain breaks with alkaline phosphatase and by centrifugation in sucrose density gradient, statistically significant differences were found between the action of active and inactivated SOD which were not observed with respect to double breaks. (author)

Additional details

Additional titles

Subtitle (English)
Studies of DNA strand breaks by ultracentrifugation and determination of terminal phosphate groups

Publishing Information

Journal Title
Stud. Biophys.
Journal Volume
78
Journal Issue
2
Series
Stud. Biophys.
Journal Page Range
151-156
ISSN
0081-6337

INIS

Country of Publication
Germany
Country of Input or Organization
German Democratic Republic
INIS RN
12601925
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Is Lead record
Yes
Descriptors DEI
ALKALINE PHOSPHATASE; COBALT 60; COMPARATIVE EVALUATIONS; CYSTEINE; DNA; GAMMA RADIATION; GLUTATHIONE; OXIDOREDUCTASES; PEROXIDES; RADIATION DOSES; RADIOPROTECTIVE SUBSTANCES; STRAND BREAKS; ULTRACENTRIFUGATION
Descriptors DEC
AMINO ACIDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; CENTRIFUGATION; COBALT ISOTOPES; DRUGS; ELECTROMAGNETIC RADIATION; ENZYMES; ESTERASES; HYDROLASES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MINUTES LIVING RADIOISOTOPES; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; OXYGEN COMPOUNDS; PEPTIDES; PHOSPHATASES; POLYPEPTIDES; PROTEINS; RADIATIONS; RADIOISOTOPES; RESPONSE MODIFYING FACTORS; SEPARATION PROCESSES; THIOLS; YEARS LIVING RADIOISOTOPES